{"id":16624,"date":"2019-09-27T12:12:27","date_gmt":"2019-09-27T11:12:27","guid":{"rendered":"http:\/\/k2info.w.uib.no\/?p=16624"},"modified":"2019-09-27T12:35:03","modified_gmt":"2019-09-27T11:35:03","slug":"nye-muligheter-for-enkeltcellestudier-ved-k2","status":"publish","type":"post","link":"https:\/\/k2info.w.uib.no\/nb_no\/2019\/09\/27\/nye-muligheter-for-enkeltcellestudier-ved-k2\/","title":{"rendered":"[:no]Nye muligheter for enkeltcellestudier ved K2[:en]Single cell studies, new technique established at K2[:]"},"content":{"rendered":"

[:no]\"\"<\/a>\u00abTo study human biology, we must know our cells. Human physiology emerges from normal cellular functions and intercellular interactions. Human disease entails the disruption of these processes\u00bb Sitatet er hentet fra presentasjonen av \u00abThe Human Cell Atlas\u00bb i eLife<\/a>. The Human Cell Atlas Consortium er et internasjonalt samarbeidsprosjekt som har som m\u00e5l \u00e5 beskrive alle typer humane celler og koble denne informasjonen med klassisk cellebiologisk kunnskap som lokalisasjon og morfologi. Atlaset skal v\u00e6re \u00e5pent tilgjengelig for alle forskere. I juni bevilget Chan Zuckerberg initiativet 68 millioner dollar til 38 forskningsgrupper for \u00e5 studere ulike organer og systemer (som immunsystemet) i ulike populasjoner ved hjelp av pr\u00f8ver fra blod og organer (organdonorer, operasjonsvev). De neste \u00e5rene vil det trolig bli generert enormt mye ny informasjon om v\u00e5re celletyper og deres funksjon og interaksjon med andre celler.<\/p>\n

Noen av forskningsgruppene p\u00e5 K2 har startet \u00e5 ta i bruk enkeltcellemetoder, men n\u00e5 blir dette mer tilgjengelig. Ni forskningsgrupper ved fakultetet har spleiset p\u00e5 innkj\u00f8p av 10X Genomics-teknologien som gj\u00f8r det mulig \u00e5 studere opp til 10\u00a0000 enkelt celler i ett fors\u00f8k. Teknikken baserer seg p\u00e5 isolering av enkeltceller i oljedr\u00e5per som ogs\u00e5 inneholder barkodete primersekvenser. Deretter bulksekvenserer man alle cellene og kan dermed se hvilke gener som uttrykkes i hver enkelt celle, noe som kan brukes til \u00e5 konstruere et vevskart der cellene grupperes etter egenskaper. I tillegg til RNA-seq kan 10X analysere kopinummervariasjon, ATAC-sekvensering p\u00e5 enkeltceller, og lange genom- og eksomsekvenseringer (https:\/\/www.10xgenomics.com\/<\/a>). Teknikken \u00e5pner for et vel av muligheter til \u00e5 studere sykdomsprosesser p\u00e5 enkeltcelleniv\u00e5 og teste ut ulike behandlinger. Et n\u00e6rliggende eksempel er kreftbehandling og behandlingsresponser til ulike tumorceller.<\/p>\n

10X-maskinen vil bli lokalisert p\u00e5 Flowplattformen og det bli gitt oppl\u00e6ring i bruken. Interessenter kan kontakte Flowplattformen for n\u00e6rmere informasjon. Forh\u00e5pentligvis vil mange grupper benytte seg av enkeltcelleteknikker.<\/p>\n

Lykke til<\/p>\n

Eystein Husebye[:en]\"\"<\/a>The Human Cell Atlas Project is an international collaborative effort that aims to define all human cell types in terms of distinctive molecular profiles (such as gene expression profiles) and to connect this information with classical cellular descriptions (such as location and morphology).\u00a0This quote is from the presentation of «The Human Cell Atlas» in eLife.<\/a>\u00a0The Human Cell Atlas Consortium is an international collaborative project aimed at describing all types of human cells and linking this information with classical cell biology knowledge such as localization and morphology. The atlas will be openly available to all researchers. In June, The Chan Zuckerberg Initiative granted $ 68 million to 38 research groups around the world to study various organs and systems (like the immune system) in different populations using blood and organ specimens (organ donors, surgical tissue). In the next few years a host of information will be available to increase our understanding of normal physiology and pathology.<\/p>\n

Some of the research groups at K2 have started using these methods, but now nine different groups at our faculty have pitched in money to buy the10X Genomics technology, making it easier to get started with single-cell studies. With 10x technology, up to 10,000 single cells can be studied in one experiment. The technique is based on the isolation of single cells in oil droplets together with a package of barcoded primers. In addition to RNA-seq 10x performs copy number profiling, ATAC sequencing at single cell resolution and long read genome- and exome sequencing (https:\/\/www.10xgenomics.com\/<\/a>). This enables one to extract information about the individual cell after bulk sequencing of the sample, and for example construct a tissue map where the cells are grouped according to properties. The technique opens up a wealth of opportunities to study disease processes at the single cell level and test out various treatments. A nearby example is cancer treatment and treatment responses to various tumor cells.<\/p>\n

The 10X machine will be located on the Flow Cytometry Core Facility at K2. Those interested can contact the Core Facility for further information. I hope that many groups will seize the opportunity to use single cell techniques.<\/p>\n

Good luck<\/p>\n

Eystein Husebye[:]<\/p>\n","protected":false},"excerpt":{"rendered":"

[:no]\u00abTo study human biology, we must know our cells. Human physiology emerges from normal cellular functions and intercellular interactions. Human disease entails the disruption of these processes\u00bb Sitatet er hentet fra presentasjonen av \u00abThe Human Cell Atlas\u00bb i eLife. The…<\/p>\n

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